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Tissue Culture Media

The number of different culture media used in micropropagation is myriad. The presence of macronutrients, micronutrients, vitamins, amino acids, a carbohydrate source, growth regulators and some kind of support are common to all media. Many micropropagation media used today are modifications of the Murashige-Skoog medium developed for tobacco tissue culture in the early 1960's. Most modified media differ in the types and concentrations of growth regulators (cytokinins, auxins) added and the type of carbohydrate source (sucrose, glucose, fructose). Auxins and cytokinins are added to culture media to induce organ formation. Cytokinins, such as kinetin (6-Furfurylaminopurine), BAP (N6­Benzylaminopurine), 2iP (6-[3-methyl-2-buten-1-ylamino)-purine, zeatin, and Thidiazuron ((N-Phenyl-N-1,2,3-Thiadiazol-5-ylurea) are added to stimulate the formation and multiplication of shoots. Auxins, such as IAA (Indole-3-acetic acid), IBA (Indole-3-butanoic acid or Indole-3-butyric acid), NAA ("-Naphthaleneacetic acid), 2,4­D (2,4-Dichlorophenoxyacetic acid) and NOA (Naphthoxyacetic acid) are added to stimulate the formation of adventitious roots.

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